6XG4
X-ray structure of Escherichia coli dihydrofolate reductase L28R mutant in complex with trimethoprim
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Dihydrofolate reductase | polymer | 165 | 18892.3 | 1 | UniProt (P0ABQ4) Pfam (PF00186) | Escherichia coli | |
| 2 | B (A) | NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 745.4 | 1 | Chemie (NDP) | |||
| 3 | C (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 4 | D (A) | TRIMETHOPRIM | non-polymer | 290.3 | 1 | Chemie (TOP) | |||
| 5 | E (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 6 | F (A) | water | water | 18.0 | 131 | Chemie (HOH) |
Sequence modifications
A: 1 - 159 (UniProt: P0ABQ4)
| PDB | External Database | Details |
|---|---|---|
| Arg 28 | Leu 28 | engineered mutation |
| His 160 | - | expression tag |
| His 161 | - | expression tag |
| His 162 | - | expression tag |
| His 163 | - | expression tag |
| His 164 | - | expression tag |
| His 165 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 18892.3 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1163.3 | |
| All* | Total formula weight | 20055.5 |
