Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004146 | molecular_function | dihydrofolate reductase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005542 | molecular_function | folic acid binding |
| A | 0005829 | cellular_component | cytosol |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0009410 | biological_process | response to xenobiotic stimulus |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0031427 | biological_process | response to methotrexate |
| A | 0046452 | biological_process | dihydrofolate metabolic process |
| A | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| A | 0046655 | biological_process | folic acid metabolic process |
| A | 0046677 | biological_process | response to antibiotic |
| A | 0050661 | molecular_function | NADP binding |
| A | 0051870 | molecular_function | methotrexate binding |
| A | 0051871 | molecular_function | dihydrofolic acid binding |
| A | 0070401 | molecular_function | NADP+ binding |
| A | 0070402 | molecular_function | NADPH binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 29 |
| Details | binding site for residue NDP A 301 |
| Chain | Residue |
| A | ALA6 |
| A | HIS45 |
| A | THR46 |
| A | LEU62 |
| A | SER63 |
| A | SER64 |
| A | LYS76 |
| A | VAL78 |
| A | ILE94 |
| A | GLY96 |
| A | GLY97 |
| A | ALA7 |
| A | ARG98 |
| A | VAL99 |
| A | TYR100 |
| A | GLN102 |
| A | TOP303 |
| A | HOH407 |
| A | HOH427 |
| A | HOH432 |
| A | HOH433 |
| A | HOH446 |
| A | ILE14 |
| A | GLY15 |
| A | ASN18 |
| A | ALA19 |
| A | MET20 |
| A | GLY43 |
| A | ARG44 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | binding site for residue GOL A 302 |
| Chain | Residue |
| A | VAL10 |
| A | ASP116 |
| A | SER148 |
| A | HIS149 |
| A | SER150 |
| A | HOH408 |
| A | HOH434 |
| site_id | AC3 |
| Number of Residues | 14 |
| Details | binding site for residue TOP A 303 |
| Chain | Residue |
| A | ILE5 |
| A | ALA6 |
| A | MET20 |
| A | ASP27 |
| A | PHE31 |
| A | SER49 |
| A | ILE50 |
| A | ILE94 |
| A | TYR100 |
| A | NDP301 |
| A | HOH409 |
| A | HOH440 |
| A | HOH470 |
| A | HOH520 |
| site_id | AC4 |
| Number of Residues | 2 |
| Details | binding site for residue CL A 304 |
| Chain | Residue |
| A | ARG98 |
| A | GLN102 |
Functional Information from PROSITE/UniProt
| site_id | PS00075 |
| Number of Residues | 23 |
| Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. VIGmenaMPWnlpa.DrawFkrnT |
| Chain | Residue | Details |
| A | VAL13-THR35 | |
Functional Information from SwissProt/UniProt
| Chain | Residue | Details |
| A | THR113 | |
| A | ILE5 | |
| A | ASP27 | |
| A | ARG52 | |
| A | ARG57 | |
| Chain | Residue | Details |
| A | VAL13 | |
| A | HIS45 | |
| A | SER63 | |
| A | LYS76 | |
| A | GLY95 | |
| A | ALA7 | |
