6XDS

Crystal structure of MBP-TREM2 Ig domain fusion with fragment, 2-((4-bromophenyl)amino)ethan-1-ol

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Sugar ABC transporter substrate-binding protein,Triggering receptor expressed on myeloid cells 2	polymer	492	54172.1	1	UniProt (A0A0F8NYV9) UniProt (Q9NZC2) Pfam (PF01547) Pfam (PF07686) UniProt (by SIFTS) (P0AEX9)	Methanosarcina mazei	TREM-2,Triggering receptor expressed on monocytes 2
2	B (B)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		504.4	1	In PDB BIRD (PRD_900009) GlyTouCan (G96370VA)		alpha-maltotriose
3	C (A)	2-[(4-bromophenyl)amino]ethan-1-ol	non-polymer		216.1	1	Chemie (V5S)
4	D, E, F (A)	DIMETHYL SULFOXIDE	non-polymer		78.1	3	Chemie (DMS)
5	G (A)	water	water		18.0	528	Chemie (HOH)

Sequence modifications

A: 1 - 367 (UniProt: A0A0F8NYV9)

PDB	External Database	Details
Ala 82	Asp 83	conflict
Ala 83	Lys 84	conflict
Ala 172	Glu 173	conflict
Ala 173	Asn 174	conflict
His 215	Ala 216	conflict
His 219	Lys 220	conflict
Ala 239	Lys 240	conflict
Val 312	Ala 313	conflict
Val 317	Ile 318	conflict
Ala 359	Glu 360	conflict
Ala 362	Lys 363	conflict
Ala 363	Asp 364	conflict

A: 368 - 485 (UniProt: Q9NZC2)

PDB	External Database	Details
Asp 370	Asn 20	conflict
His 486	-	expression tag
His 487	-	expression tag
His 488	-	expression tag
His 489	-	expression tag
His 490	-	expression tag
His 491	-	expression tag
His 492	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	54172.1
Branched	Number of molecules	1
	Total formula weight	504.4
Non-Polymers*	Number of molecules	4
	Total formula weight	450.5
All*	Total formula weight	55127.0

*Water molecules are not included.