6XDS
Crystal structure of MBP-TREM2 Ig domain fusion with fragment, 2-((4-bromophenyl)amino)ethan-1-ol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.916360 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.840, 80.350, 103.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.440 - 1.466 |
R-factor | 0.189 |
Rwork | 0.188 |
R-free | 0.20820 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | BUSTER |
Refinement software | BUSTER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.440 | 1.608 |
High resolution limit [Å] | 1.466 | 1.466 |
Number of reflections | 76286 | 3814 |
<I/σ(I)> | 14.7 | |
Completeness [%] | 72.3 | 15.5 |
Redundancy | 6.6 | 6 |
CC(1/2) | 0.999 | 0.572 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 100 mM BisTris pH 5.5, 100 mM ammonium acetate, 15% PEG4000, 15% glycerol |