6XBM

Structure of human SMO-Gi complex with 24(S),25-EC

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Smoothened homolog	polymer	652	71786.4	1	UniProt (Q99835) Pfam (PF01392) Pfam (PF01534)	Homo sapiens (Human)	SMO,Protein Gx
2	B (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40415.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	344	37671.1	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (Human)	Transducin beta chain 1
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
5	E (S)	scFv16	polymer	259	27784.9	1		Mus musculus
6	F, G (R)	17-[3-(3,3-DIMETHYL-OXIRANYL)-1-METHYL-PROPYL]-10,13-DIMETHYL-2,3,4,7,8,9,10,11,12,13,14,15,16,17-TETRADECAHYDRO-1H-CYC LOPENTA[A]PHENANTHREN-3-OL	non-polymer		400.6	2	Chemie (CO1)

Sequence modifications

R: 1 - 644 (UniProt: Q99835)

PDB	External Database	Details
Asp 645	-	expression tag
Tyr 646	-	expression tag
Lys 647	-	expression tag
Asp 648	-	expression tag
Asp 649	-	expression tag
Asp 650	-	expression tag
Asp 651	-	expression tag
Lys 652	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	185518.6
Non-Polymers*	Number of molecules	2
	Total formula weight	801.3
All*	Total formula weight	186319.9

*Water molecules are not included.