6XAS
CryoEM Structure of E. coli Rho-dependent Transcription Pre-termination Complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (G) | Transcription termination/antitermination protein NusA | polymer | 495 | 54932.7 | 1 | UniProt (P0AFF6) Pfam (PF08529) Pfam (PF00575) Pfam (PF13184) Pfam (PF14520) | Escherichia coli (strain K12) | N utilization substance protein A,Transcription termination/antitermination L factor |
| 2 | B (W) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli (strain K12) | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 3 | C (N) | DNA (29-MER) | polymer | 29 | 8840.7 | 1 | Escherichia coli (strain K12) | ||
| 4 | D (T) | DNA (29-MER) | polymer | 29 | 8813.6 | 1 | Escherichia coli (strain K12) | ||
| 5 | E (R) | RNA (60-MER) | polymer | 60 | 19051.4 | 1 | Escherichia coli (strain K12) | ||
| 6 | F, G (K, H) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF03118) Pfam (PF01193) Pfam (PF01000) | Escherichia coli (strain K12) | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 7 | H (I) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) | Escherichia coli (strain K12) | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 8 | I (J) | DNA-directed RNA polymerase subunit beta' | polymer | 1416 | 156505.0 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Escherichia coli (strain K12) | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 9 | J, K, L, M, N... (C, A, B, D, E...) | Transcription termination factor Rho | polymer | 419 | 47070.2 | 6 | UniProt (P0AG30) Pfam (PF07498) Pfam (PF07497) Pfam (PF00006) | Escherichia coli (strain K12) | ATP-dependent helicase Rho |
| 10 | P (J) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 11 | Q, R (J) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
J: 2 - 1407 (UniProt: P0A8T7)
| PDB | External Database | Details |
|---|---|---|
| Val 1 | - | expression tag |
| Leu 1408 | - | expression tag |
| Glu 1409 | - | expression tag |
| Val 1410 | - | expression tag |
| His 1411 | - | expression tag |
| His 1412 | - | expression tag |
| His 1413 | - | expression tag |
| His 1414 | - | expression tag |
| His 1415 | - | expression tag |
| His 1416 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 15 |
| Total formula weight | 764752.2 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 764907.3 |
