6X9O

High resolution cryoEM structure of huntingtin in complex with HAP40

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Huntingtin	polymer	3156	349472.3	1	UniProt (P42858) Pfam (PF20926) Pfam (PF20925) Pfam (PF20927) In PDB	Homo sapiens (Human)	Huntington disease protein,HD protein
2	B	40-kDa huntingtin-associated protein	polymer	389	41342.3	1	UniProt (P23610) In PDB	Homo sapiens (Human)	HAP40,CpG island protein,Factor VIII intron 22 protein

Sequence modifications

A: 1 - 3144 (UniProt: P42858)

PDB	External Database	Details
Gln 39	-	insertion
Gln 40	-	insertion
His 2311	Tyr 2309	variant
Ile 2788	Val 2786	variant
Gly 3145	-	expression tag
Gly 3146	-	expression tag
Ser 3147	-	expression tag
Gly 3148	-	expression tag
Asp 3149	-	expression tag
Tyr 3150	-	expression tag
Lys 3151	-	expression tag
Asp 3152	-	expression tag
Asp 3153	-	expression tag
Asp 3154	-	expression tag
Asp 3155	-	expression tag
Lys 3156	-	expression tag

B: 1 - 371 (UniProt: P23610)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Arg -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	390814.6
All*	Total formula weight	390814.6

*Water molecules are not included.