6X90

Structure of the guanine nucleotide exchange factor Sec12 bound to the small GTPase Sar1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Guanine nucleotide-exchange factor SEC12	polymer	360	39369.7	1	UniProt (P11655) Pfam (PF00400)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Protein transport protein SEC12
2	B (A)	Small COPII coat GTPase SAR1	polymer	173	19596.3	1	UniProt (P20606) Pfam (PF00025)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	GTP-binding protein SAR1,Secretion-associated RAS-related protein 1
3	C (B)	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
4	D, E (B, A)	water	water		18.0	72	Chemie (HOH)

Sequence modifications

B: 1 - 354 (UniProt: P11655)

PDB	External Database	Details
His 355	-	expression tag
His 356	-	expression tag
His 357	-	expression tag
His 358	-	expression tag
His 359	-	expression tag
His 360	-	expression tag

A: 24 - 190 (UniProt: P20606)

PDB	External Database	Details
His 191	-	expression tag
His 192	-	expression tag
His 193	-	expression tag
His 194	-	expression tag
His 195	-	expression tag
His 196	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58966.0
Non-Polymers*	Number of molecules	1
	Total formula weight	39.1
All*	Total formula weight	59005.1

*Water molecules are not included.