6X90
Structure of the guanine nucleotide exchange factor Sec12 bound to the small GTPase Sar1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.333, 93.620, 115.423 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.710 | 2.330 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.039 | 0.741 |
Rmeas | 0.043 | 0.806 |
Rpim | 0.017 | 0.313 |
Number of reflections | 20215 | 1012 |
<I/σ(I)> | 20.1 | 2.4 |
Completeness [%] | 95.3 | 98.6 |
Redundancy | 6.5 | 6.4 |
CC(1/2) | 1.000 | 0.854 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 5mg/mL protein, 100mM MES pH6.5, 30% PEG 5000 MME |