6X61

Crystal structure of the N-terminal thioredoxin domain of SasA in complex with the N-terminal CI domain of KaiC from Thermosynchococcus elongatus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I... (A, C, E, G, I...)	Circadian clock protein kinase KaiC	polymer	247	27738.2	6	UniProt (Q79V60) Pfam (PF06745)	Thermosynechococcus elongatus
2	B, D, F, H, J... (B, D, F, H, J...)	Adaptive-response sensory-kinase SasA	polymer	108	12629.2	6	UniProt (Q8DMT2) Pfam (PF07689)	Thermosynechococcus elongatus
3	M, N, O, P, Q... (A, C, E, G, I...)	PHOSPHATE ION	non-polymer		95.0	6	Chemie (PO4)

Sequence modifications

A, C, E, G, I, K: 17 - 247 (UniProt: Q79V60)

PDB	External Database	Details
Asp 9	-	expression tag
Tyr 10	-	expression tag
Lys 11	-	expression tag
Asp 12	-	expression tag
Asp 13	-	expression tag
Asp 14	-	expression tag
Asp 15	-	expression tag
Lys 16	-	expression tag
Ala 41	Arg 41	engineered mutation
Ala 173	Lys 173	engineered mutation
Asp 248	-	expression tag
Tyr 249	-	expression tag
Lys 250	-	expression tag
Asp 251	-	expression tag
Asp 252	-	expression tag
Asp 253	-	expression tag
Asp 254	-	expression tag
Lys 255	-	expression tag

B, D, F, H, J, L: 16 - 107 (UniProt: Q8DMT2)

PDB	External Database	Details
Asp 8	-	expression tag
Tyr 9	-	expression tag
Lys 10	-	expression tag
Asp 11	-	expression tag
Asp 12	-	expression tag
Asp 13	-	expression tag
Asp 14	-	expression tag
Lys 15	-	expression tag
Ala 16	Pro 16	engineered mutation
Asp 108	-	expression tag
Tyr 109	-	expression tag
Lys 110	-	expression tag
Asp 111	-	expression tag
Asp 112	-	expression tag
Asp 113	-	expression tag
Asp 114	-	expression tag
Lys 115	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	242204.3
Non-Polymers*	Number of molecules	6
	Total formula weight	569.8
All*	Total formula weight	242774.1

*Water molecules are not included.