6X61

Crystal structure of the N-terminal thioredoxin domain of SasA in complex with the N-terminal CI domain of KaiC from Thermosynchococcus elongatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0331
Spacegroup name	P 1 21 1
Unit cell lengths	107.600, 121.580, 133.590
Unit cell angles	90.00, 108.78, 90.00

Refinement procedure

Resolution	49.049 - 3.200
R-factor	0.2239
Rwork	0.222
R-free	0.26530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jwo
RMSD bond length	0.003
RMSD bond angle	0.778
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.050	3.300
High resolution limit [Å]	3.200	3.200
Rmerge	0.160	0.750
Number of reflections	53267	4635
<I/σ(I)>	5.5	1.5
Completeness [%]	98.8
Redundancy	2.9
CC(1/2)	0.950	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	1.26 M NaH2PO4, 0.54 M K2HPO4 (pH unadjusted), 0.1 M Glycine pH 10.5, 0.2 M Li2SO4, 22 degrees C