6X4G

Crystal structure of ICOS in complex with ICOS-L and an anti ICOS-L VNAR domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Inducible T-cell costimulator	polymer	119	13412.2	1	UniProt (Q9Y6W8) Pfam (PF15910) In PDB	Homo sapiens (Human)	Activation-inducible lymphocyte immunomediatory molecule
2	C	ICOS ligand	polymer	240	26771.8	1	UniProt (O75144) Pfam (PF07686) In PDB	Homo sapiens (Human)	B7 homolog 2,B7-H2,B7-like protein Gl50,B7-related protein 1,B7RP-1
3	N	anti ICOS-L VHH domain VNAR	polymer	130	13987.4	1		Orectolobus maculatus
4	B, D	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	2	In PDB GlyTouCan (G22768VO)
5	A, C	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)
6	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)

Sequence modifications

A: 19 - 129 (UniProt: Q9Y6W8)

PDB	External Database	Details
Gly 130	-	expression tag
Thr 131	-	expression tag
Glu 132	-	expression tag
Asn 133	-	expression tag
Leu 134	-	expression tag
Tyr 135	-	expression tag
Phe 136	-	expression tag
Gln 137	-	expression tag

C: 19 - 248 (UniProt: O75144)

PDB	External Database	Details
Thr 17	-	cloning artifact
Gly 18	-	cloning artifact
Gly 249	-	expression tag
Thr 250	-	expression tag
Glu 251	-	expression tag
Asn 252	-	expression tag
Leu 253	-	expression tag
Tyr 254	-	expression tag
Phe 255	-	expression tag
Gln 256	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	54171.5
Branched	Number of molecules	2
	Total formula weight	1821.6
Non-Polymers*	Number of molecules	7
	Total formula weight	1419.3
All*	Total formula weight	57412.4

*Water molecules are not included.