6X4G
Crystal structure of ICOS in complex with ICOS-L and an anti ICOS-L VNAR domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 104.015, 104.015, 123.108 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.730 - 3.500 |
R-factor | 0.2262 |
Rwork | 0.224 |
R-free | 0.26650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i8l 4i0k 1t6v |
RMSD bond length | 0.010 |
RMSD bond angle | 1.131 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.270 | 0.790 |
Rpim | 0.079 | 0.230 |
Number of reflections | 10227 | 406 |
<I/σ(I)> | 10 | 1.7 |
Completeness [%] | 95.7 | 97.9 |
Redundancy | 11.8 | 12.2 |
CC(1/2) | 0.990 | 0.630 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M HEPES, pH 7.0, and 30% (v/v) Jeffamine ED-2001 |