6X1G

Crystal structure of a GEF domain from the Orientia tsutsugamushi protein OtDUB in complex with Rac1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, C)	ULP_PROTEASE domain-containing protein	polymer	219	25190.5	2	UniProt (B3CVM3)	Orientia tsutsugamushi (Rickettsia tsutsugamushi)
2	C, D (B, D)	Ras-related C3 botulinum toxin substrate 1	polymer	183	20539.6	2	UniProt (P63000) Pfam (PF00071)	Homo sapiens (Human)	Rac1, Cell migration-inducing gene 5 protein,Ras-like protein TC25,p21-Rac1
3	E (C)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	F, G, H, I (A, C, B, D)	water	water		18.0	311	Chemie (HOH)

Sequence modifications

A, C: 548 - 759 (UniProt: B3CVM3)

PDB	External Database	Details
Met 547	-	initiating methionine
His 760	-	expression tag
His 761	-	expression tag
His 762	-	expression tag
His 763	-	expression tag
His 764	-	expression tag
His 765	-	expression tag

B, D: 1 - 177 (UniProt: P63000)

PDB	External Database	Details
His 178	-	expression tag
His 179	-	expression tag
His 180	-	expression tag
His 181	-	expression tag
His 182	-	expression tag
His 183	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	91460.4
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	91484.7

*Water molecules are not included.