6X1B

Crystal Structure of NSP15 Endoribonuclease from SARS CoV-2 in the Complex with the Product Nucleotide GpU.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Uridylate-specific endoribonuclease	polymer	370	41657.2	2	UniProt (P0DTD1) Pfam (PF19219) Pfam (PF19216) Pfam (PF19215)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	pp1ab,ORF1ab polyprotein,NendoU,Non-structural protein 15,nsp15
2	C, D (D, F)	DNA (5'-R(*GP*U)-3')	polymer	2	606.4	2		synthetic construct
3	E, I (A, B)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
4	F, G, H (B)	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
5	J, K, L, M (A, B, D, F)	water	water		18.0	432	Chemie (HOH)

Sequence modifications

A, B: 2 - 347 (UniProt: P0DTD1)

PDB	External Database	Details
Met -22	-	initiating methionine
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
Gly -13	-	expression tag
Val -12	-	expression tag
Asp -11	-	expression tag
Leu -10	-	expression tag
Gly -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Ser -1	-	expression tag
Asn 0	-	expression tag
Met 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	84527.3
Non-Polymers*	Number of molecules	5
	Total formula weight	376.1
All*	Total formula weight	84903.4

*Water molecules are not included.