6X1B
Crystal Structure of NSP15 Endoribonuclease from SARS CoV-2 in the Complex with the Product Nucleotide GpU.
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 9 |
| Details | binding site for residue PO4 A 401 |
| Chain | Residue |
| A | HIS235 |
| A | GLY248 |
| A | HIS250 |
| A | LYS290 |
| A | THR341 |
| A | HOH560 |
| A | HOH567 |
| D | G401 |
| D | U402 |
| site_id | AC2 |
| Number of Residues | 8 |
| Details | binding site for residue EDO B 501 |
| Chain | Residue |
| A | LYS320 |
| A | THR322 |
| A | GLU327 |
| B | VAL149 |
| B | LYS150 |
| B | GLY151 |
| B | LEU152 |
| B | HOH745 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | binding site for residue EDO B 502 |
| Chain | Residue |
| B | THR113 |
| B | GLU114 |
| B | HOH678 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | binding site for residue EDO B 503 |
| Chain | Residue |
| B | SER244 |
| B | GLN245 |
| B | SER289 |
| B | HOH610 |
| B | HOH615 |
| site_id | AC5 |
| Number of Residues | 9 |
| Details | binding site for residue PO4 B 504 |
| Chain | Residue |
| B | HIS235 |
| B | GLY248 |
| B | HIS250 |
| B | LYS290 |
| B | THR341 |
| B | TYR343 |
| F | G401 |
| F | U402 |
| F | HOH502 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton donor; for uridylate-specific endoribonuclease nsp15 activity => ECO:0000269|PubMed:33504779, ECO:0000269|PubMed:33564093 |
| Chain | Residue | Details |
| A | HIS235 | |
| B | HIS235 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor; for uridylate-specific endoribonuclease nsp15 activity => ECO:0000269|PubMed:33504779, ECO:0000269|PubMed:33564093 |
| Chain | Residue | Details |
| A | HIS250 | |
| B | HIS250 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | ACT_SITE: For uridylate-specific endoribonuclease nsp15 activity => ECO:0000269|PubMed:33504779 |
| Chain | Residue | Details |
| A | LYS290 | |
| B | LYS290 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:33504779, ECO:0000269|PubMed:33564093 |
| Chain | Residue | Details |
| A | LYS290 | |
| B | LYS290 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:33564093 |
| Chain | Residue | Details |
| A | THR341 | |
| B | THR341 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | SITE: Transition state stabilizer => ECO:0000269|PubMed:33504779, ECO:0000269|PubMed:33564093 |
| Chain | Residue | Details |
| A | LYS290 | |
| B | LYS290 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | SITE: Uracil recognition site => ECO:0000269|PubMed:33504779, ECO:0000269|PubMed:33564093 |
| Chain | Residue | Details |
| A | SER294 | |
| B | SER294 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | SITE: Cleavage; by 3CL-PRO => ECO:0000250|UniProtKB:P0C6V3 |
| Chain | Residue | Details |
| A | GLN347 | |
| B | GLN347 |
