6WZ4
Complex of mutant (K173M) of Pseudomonas 7A Glutaminase-Asparaginase with L-Asp at pH 6. Covalent acyl-enzyme intermediate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Glutaminase-asparaginase | polymer | 337 | 36198.0 | 4 | UniProt (Q88K39) Pfam (PF00710) Pfam (PF17763) | Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) | L-ASNase/L-GLNase,L-asparagine/L-glutamine amidohydrolase |
| 2 | E, F, G, H (A, B, C, D) | ASPARTIC ACID | non-polymer | 133.1 | 4 | Chemie (ASP) | |||
| 3 | I, J, K, L (A, B, C, D) | water | water | 18.0 | 1124 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 337 (UniProt: Q88K39)
| PDB | External Database | Details |
|---|---|---|
| Met 173 | Lys 198 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 144792.0 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 532.4 | |
| All* | Total formula weight | 145324.4 |
