6WZ4
Complex of mutant (K173M) of Pseudomonas 7A Glutaminase-Asparaginase with L-Asp at pH 6. Covalent acyl-enzyme intermediate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.034, 129.754, 81.409 |
Unit cell angles | 90.00, 118.72, 90.00 |
Refinement procedure
Resolution | 33.140 - 1.580 |
R-factor | 0.139 |
Rwork | 0.139 |
R-free | 0.15680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pga |
RMSD bond length | 0.013 |
RMSD bond angle | 1.804 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.140 | 1.620 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.088 | 0.725 |
Rpim | 0.051 | 0.453 |
Number of reflections | 191717 | 9296 |
<I/σ(I)> | 14.9 | 2.1 |
Completeness [%] | 98.7 | 96.4 |
Redundancy | 3.8 | 3.3 |
CC(1/2) | 0.990 | 0.654 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 8% w/v Tacsimate, pH 6.0, 20% w/v PEG3350 |