6WZ3

Cu-bound structure of the engineered protein trimer, TriCyt3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Soluble cytochrome b562	polymer	106	11796.4	3	UniProt (P0ABE7) Pfam (PF07361) In PDB	Escherichia coli	Cytochrome b-562
2	A, B, C	HEME C	non-polymer		618.5	3	Chemie (HEC)
3	A, B, C	CHLORIDE ION	non-polymer		35.5	7	Chemie (CL)
4	A	COPPER (II) ION	non-polymer		63.5	1	Chemie (CU)
5	B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
6		water	water		18.0	306	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 106 (UniProt: P0ABE7)

PDB	External Database	Details
Lys 31	Thr 53	engineered mutation
Lys 35	Ala 57	engineered mutation
Lys 41	Gln 63	engineered mutation
Ala 54	Asp 76	engineered mutation
Ile 59	Lys 81	engineered mutation
Val 63	His 85	engineered mutation
Glu 67	Ile 89	engineered mutation
Ala 69	Val 91	engineered mutation
Trp 70	Gly 92	engineered mutation
Glu 71	Gln 93	engineered mutation
His 73	Asp 95	engineered mutation
Ala 76	Leu 98	engineered mutation
His 77	Lys 99	engineered mutation
Lys 80	Asn 102	engineered mutation
Cys 98	Arg 120	engineered mutation
Cys 101	Tyr 123	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	35389.2
Non-Polymers*	Number of molecules	12
	Total formula weight	2273.3
All*	Total formula weight	37662.6

*Water molecules are not included.