6WZ3
Cu-bound structure of the engineered protein trimer, TriCyt3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.485500 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 78.447, 81.205, 56.431 |
Unit cell angles | 90.00, 92.92, 90.00 |
Refinement procedure
Resolution | 39.182 - 1.800 |
R-factor | 0.1905 |
Rwork | 0.188 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bc5 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.801 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.182 | 1.864 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.034 | 0.281 |
Rmeas | 0.041 | 0.344 |
Rpim | 0.022 | 0.195 |
Number of reflections | 61197 | 3241 |
<I/σ(I)> | 22.13 | 3.16 |
Completeness [%] | 94.9 | |
Redundancy | 3.2 | |
CC(1/2) | 0.999 | 0.891 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 25% PEG1500, 0.2 M MgCl2, 0.1 M BIS-TRIS (pH 5.5) |