6WW2
Structure of human Frizzled5 by fiducial-assisted cryo-EM
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | H | anti-BRIL Fab Heavy chain | polymer | 229 | 24321.1 | 1 | Homo sapiens (human) | ||
| 2 | K | anti-Fab Nanobody | polymer | 123 | 13390.6 | 1 | synthetic construct | ||
| 3 | L | anti-BRIL Fab Light chain | polymer | 214 | 23353.9 | 1 | Homo sapiens (human) | ||
| 4 | R | Frizzled-5,Soluble cytochrome b562 | polymer | 667 | 74754.2 | 1 | UniProt (Q13467) UniProt (P0ABE7) Pfam (PF01392) Pfam (PF01534) Pfam (PF07361) In PDB | Homo sapiens (Human) | hFz5,FzE5,Cytochrome b-562,hFz5,FzE5 |
Sequence modifications
R: 27 - 428 (UniProt: Q13467)
R: 1006 - 1110 (UniProt: P0ABE7)
R: 441 - 546 (UniProt: Q13467)
| PDB | External Database | Details |
|---|---|---|
| Asp 20 | - | expression tag |
| Tyr 21 | - | expression tag |
| Lys 22 | - | expression tag |
| Asp 23 | - | expression tag |
| Asp 24 | - | expression tag |
| Asp 25 | - | expression tag |
| Asp 26 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Trp 1012 | Met 29 | conflict |
| Ile 1107 | His 124 | conflict |
| Leu 1111 | - | linker |
| Glu 1112 | - | linker |
| Arg 1113 | - | linker |
| Ala 1114 | - | linker |
| Arg 1115 | - | linker |
| Ser 1116 | - | linker |
| Thr 1117 | - | linker |
| Leu 1118 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Ala 1001 | - | linker |
| Arg 1002 | - | linker |
| Arg 1003 | - | linker |
| Gln 1004 | - | linker |
| Leu 1005 | - | linker |
| Ala 547 | - | expression tag |
| Ala 548 | - | expression tag |
| Ala 549 | - | expression tag |
| Leu 550 | - | expression tag |
| Glu 551 | - | expression tag |
| Val 552 | - | expression tag |
| Leu 553 | - | expression tag |
| Phe 554 | - | expression tag |
| Gln 555 | - | expression tag |
| Gly 556 | - | expression tag |
| Pro 557 | - | expression tag |
| Gly 558 | - | expression tag |
| Ala 559 | - | expression tag |
| Ala 560 | - | expression tag |
| Glu 561 | - | expression tag |
| Asp 562 | - | expression tag |
| Gln 563 | - | expression tag |
| Val 564 | - | expression tag |
| Asp 565 | - | expression tag |
| Pro 566 | - | expression tag |
| Arg 567 | - | expression tag |
| Leu 568 | - | expression tag |
| Ile 569 | - | expression tag |
| Asp 570 | - | expression tag |
| Gly 571 | - | expression tag |
| Lys 572 | - | expression tag |
| His 573 | - | expression tag |
| His 574 | - | expression tag |
| His 575 | - | expression tag |
| His 576 | - | expression tag |
| His 577 | - | expression tag |
| His 578 | - | expression tag |
| His 579 | - | expression tag |
| His 580 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 135819.9 | |
| All* | Total formula weight | 135819.9 |
