6WUI

Crystal Structure of mutant S. pombe Rai1 (E150S/E199Q/E239Q) in complex with 3'-FADP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Decapping nuclease din1	polymer	372	42927.0	1	UniProt (O13836) Pfam (PF08652)	Schizosaccharomyces pombe (Fission yeast)	Dhp1-interacting protein 1
2	B (A)	[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-(7,8-dimethyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl)-2,3,4-trihydroxypentyl dihydrogen diphosphate (non-preferred name)	non-polymer		865.5	1	Chemie (UBG)
3	C (A)	water	water		18.0	139	Chemie (HOH)

Sequence modifications

A: 1 - 352 (UniProt: O13836)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ser 150	Glu 150	engineered mutation
Gln 199	Glu 199	engineered mutation
Gln 239	Glu 239	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	42927.0
Non-Polymers*	Number of molecules	1
	Total formula weight	865.5
All*	Total formula weight	43792.5

*Water molecules are not included.