6WRQ
Crystal structure of Mj 3-nitro-tyrosine tRNA synthetase (5B) S158C variant bound to 3-nitro-tyrosine
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Tyrosine--tRNA ligase | polymer | 314 | 36103.9 | 1 | UniProt (Q57834) Pfam (PF00579) In PDB | Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) | Tyrosyl-tRNA synthetase,TyrRS |
2 | A | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
3 | A | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
4 | A | META-NITRO-TYROSINE | non-polymer | 226.2 | 2 | Chemie (NIY) | |||
5 | water | water | 18.0 | 211 | Chemie (HOH) |
Sequence modifications
A: 1 - 306 (UniProt: Q57834)
PDB | External Database | Details |
---|---|---|
His 32 | Tyr 32 | engineered mutation |
Cys 70 | His 70 | engineered mutation |
Cys 158 | Asp 158 | engineered mutation |
Ala 159 | Ile 159 | engineered mutation |
Arg 162 | Leu 162 | engineered mutation |
Leu 307 | - | expression tag |
Glu 308 | - | expression tag |
His 309 | - | expression tag |
His 310 | - | expression tag |
His 311 | - | expression tag |
His 312 | - | expression tag |
His 313 | - | expression tag |
His 314 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 36103.9 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 659.5 | |
All* | Total formula weight | 36763.5 |