6WPW

GCGR-Gs signaling complex bound to a designed glucagon derivative

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	380	44326.2	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein
2	D	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
4	N	Nb35	polymer	138	15140.7	1		Lama glama (llama)
5	P	Glucagon derivative ZP3780	polymer	29	3459.7	1		synthetic construct
6	R	Glucagon receptor	polymer	496	56334.9	1	UniProt (P47871) Pfam (PF02793) Pfam (PF00002) In PDB	Homo sapiens (Human)	GL-R

Sequence modifications

D: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 27 - 477 (UniProt: P47871)

PDB	External Database	Details
Met -7	-	expression tag
Lys -6	-	expression tag
Thr -5	-	expression tag
Ile -4	-	expression tag
Ile -3	-	expression tag
Ala -2	-	expression tag
Leu -1	-	expression tag
Ser 0	-	expression tag
Tyr 1	-	expression tag
Ile 2	-	expression tag
Phe 3	-	expression tag
Cys 4	-	expression tag
Leu 5	-	expression tag
Val 6	-	expression tag
Phe 7	-	expression tag
Ala 8	-	expression tag
Asp 9	-	expression tag
Tyr 10	-	expression tag
Lys 11	-	expression tag
Asp 12	-	expression tag
Asp 13	-	expression tag
Asp 14	-	expression tag
Asp 15	-	expression tag
Ala 16	-	expression tag
Leu 17	-	expression tag
Glu 18	-	expression tag
Val 19	-	expression tag
Leu 20	-	expression tag
Phe 21	-	expression tag
Gln 22	-	expression tag
Gly 23	-	expression tag
Pro 24	-	expression tag
Ser 25	-	expression tag
Gly 26	-	expression tag
Gly 478	-	expression tag
Ser 479	-	expression tag
Gly 480	-	expression tag
His 481	-	expression tag
His 482	-	expression tag
His 483	-	expression tag
His 484	-	expression tag
His 485	-	expression tag
His 486	-	expression tag
His 487	-	expression tag
His 488	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	166540.7
All*	Total formula weight	166540.7

*Water molecules are not included.