6WMN

Human poly-N-acetyl-lactosamine synthase structure demonstrates a modular assembly of catalytic subsites for GT-A glycosyltransferases

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2	polymer	364	42220.2	4	UniProt (Q9NY97) Pfam (PF01762)	Homo sapiens (Human)	Beta-1,3-N-acetylglucosaminyltransferase 1,Beta3Gn-T1,3-galactosyltransferase 7,b3Gal-T7,Beta-3-Gx-T7,UDP-Gal:beta-GlcNAc beta-1,UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2,Beta3Gn-T2,UDP-galactose:beta-N-acetylglucosamine beta-1
2	E, G, H (E, G, H)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
3	F (F)	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G22573RC)
4	I, L, P, U (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
5	J, M, Q, V (A, B, C, D)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	4	Chemie (UDP)
6	K, N, R, W (A, B, C, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
7	O, T, X (B, C, D)	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
8	S (C)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
9	AA, BA, Y, Z (C, D, A, B)	water	water		18.0	289	Chemie (HOH)

Sequence modifications

A, B, C, D: 35 - 397 (UniProt: Q9NY97)

PDB	External Database	Details
Gly 34	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	168880.8
Branched	Number of molecules	4
	Total formula weight	2508.3
Non-Polymers*	Number of molecules	16
	Total formula weight	2820.4
All*	Total formula weight	174209.5

*Water molecules are not included.