6WID
Nucleotide incorporation intermediate into quaternary complex of human Polymerase Mu on a complementary DNA double-strand break substrate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed DNA/RNA polymerase mu | polymer | 356 | 40054.4 | 1 | UniProt (Q9NP87) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) | Homo sapiens (Human) | Pol Mu,Terminal transferase |
| 2 | B (T) | DNA (5'-D(*CP*GP*GP*CP*AP*T)-3') | polymer | 6 | 1809.2 | 1 | synthetic construct | ||
| 3 | C (U) | DNA (5'-D(*AP*CP*G)-3') | polymer | 3 | 886.6 | 1 | synthetic construct | ||
| 4 | D (P) | DNA (5'-D(*CP*GP*TP*AP*T)-3') | polymer | 5 | 1495.0 | 1 | synthetic construct | ||
| 5 | E (D) | DNA (5'-D(P*GP*CP*CP*G)-3') | polymer | 4 | 1191.8 | 1 | synthetic construct | ||
| 6 | F (A) | 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE | non-polymer | 467.2 | 1 | Chemie (DUP) | |||
| 7 | G (A) | PYROPHOSPHATE | non-polymer | 178.0 | 1 | Chemie (PPV) | |||
| 8 | H, I (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 9 | J (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 10 | K (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 11 | L (A) | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 1 | Chemie (1PE) | |||
| 12 | M, N (A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 13 | O (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 14 | P, Q, R, S, T (A, T, U, P, D) | water | water | 18.0 | 350 | Chemie (HOH) |
Sequence modifications
A: 132 - 494 (UniProt: Q9NP87)
| PDB | External Database | Details |
|---|---|---|
| Gly 127 | - | expression tag |
| Ser 128 | - | expression tag |
| Ala 129 | - | expression tag |
| Ala 130 | - | expression tag |
| Ala 131 | - | expression tag |
| - | Pro 398 | deletion |
| - | Gly 399 | deletion |
| - | Ala 400 | deletion |
| - | Ala 401 | deletion |
| - | Val 402 | deletion |
| - | Gly 403 | deletion |
| - | Gly 404 | deletion |
| - | Ser 405 | deletion |
| - | Thr 406 | deletion |
| - | Arg 407 | deletion |
| - | Pro 408 | deletion |
| - | Cys 409 | deletion |
| Gly 410 | Pro 410 | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 45437.1 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1280.8 | |
| All* | Total formula weight | 46717.9 |
