6WGM

Crystal structure of a marine metagenome TRAP solute binding protein specific for pyroglutamate (Sorcerer II Global Ocean Sampling Expedition, unidentified microbe, scf7180008839099) in complex with co-purified pyroglutamate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	TRAP-type C4-dicarboxylate transport system, periplasmic component	polymer	323	35375.8	1	UniProt (G5ZWD6) Pfam (PF03480)	uncultured bacterium
2	B (A)	PYROGLUTAMIC ACID	non-polymer		129.1	1	Chemie (PCA)
3	C, D, E, F, G... (A)	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
4	I (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5	J, K, L, M, N... (A)	FORMIC ACID	non-polymer		46.0	10	Chemie (FMT)
6	T (A)	water	water		18.0	296	Chemie (HOH)

Sequence modifications

A: 24 - 323 (UniProt: G5ZWD6)

PDB	External Database	Details
Met 1	-	initiating methionine
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
Ser 8	-	expression tag
Ser 9	-	expression tag
Gly 10	-	expression tag
Val 11	-	expression tag
Asp 12	-	expression tag
Leu 13	-	expression tag
Gly 14	-	expression tag
Thr 15	-	expression tag
Glu 16	-	expression tag
Asn 17	-	expression tag
Leu 18	-	expression tag
Tyr 19	-	expression tag
Phe 20	-	expression tag
Gln 21	-	expression tag
Ser 22	-	expression tag
Met 23	-	expression tag
Asp 46	Glu 46	conflict
Asp 114	Asn 114	conflict
Ser 218	Thr 218	conflict
Asp 244	Glu 244	conflict
Ala 262	Ser 262	conflict
Asn 270	Ser 270	conflict
Ala 289	Gly 289	conflict
Ser 296	Ala 296	conflict

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	35375.8
Non-Polymers*	Number of molecules	18
	Total formula weight	997.2
All*	Total formula weight	36373.0

*Water molecules are not included.