6W9D

RNF12 RING domain in complex with a Ube2d2~Ub conjugate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G	Ubiquitin-conjugating enzyme E2 D2	polymer	155	17874.3	3	UniProt (P62837) Pfam (PF00179) In PDB	Homo sapiens (Human)	(E3-independent) E2 ubiquitin-conjugating enzyme D2,E2 ubiquitin-conjugating enzyme D2,Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1
2	B, E, H	E3 ubiquitin-protein ligase RLIM	polymer	98	11043.4	3	UniProt (Q9NVW2) Pfam (PF13639) In PDB	Homo sapiens (Human)	LIM domain-interacting RING finger protein,RING finger LIM domain-binding protein,R-LIM,RING finger protein 12,RING-type E3 ubiquitin transferase RLIM,Renal carcinoma antigen NY-REN-43, RNF12
3	C, F, I	Ubiquitin	polymer	76	8576.8	3	UniProt (P0CG48) Pfam (PF00240) In PDB	Homo sapiens (Human)
4	B, E, H	ZINC ION	non-polymer		65.4	6	Chemie (ZN)
5	B, C, D, E, F...	IODIDE ION	non-polymer		126.9	8	Chemie (IOD)

Sequence modifications

A, D, G: 1 - 147 (UniProt: P62837)

PDB	External Database	Details
Ser 21	Cys 21	engineered mutation
Arg 22	Ser 22	engineered mutation
Lys 85	Cys 85	engineered mutation
Ser 107	Cys 107	engineered mutation
Ser 111	Cys 111	engineered mutation
Leu 148	-	expression tag
Glu 149	-	expression tag
His 150	-	expression tag
His 151	-	expression tag
His 152	-	expression tag
His 153	-	expression tag
His 154	-	expression tag
His 155	-	expression tag

B, E, H: 530 - 623 (UniProt: Q9NVW2)

PDB	External Database	Details
Gly 526	-	expression tag
Pro 527	-	expression tag
Leu 528	-	expression tag
Gly 529	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	112483.7
Non-Polymers*	Number of molecules	14
	Total formula weight	1407.7
All*	Total formula weight	113891.3

*Water molecules are not included.