6W7A

The crystal structure of the 2009/H1N1/California PA endonuclease mutant E119D bound to DNA oligomer TAGCAT (uncleaved, 5mM overnight DNA soak)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Protein PA-X,Polymerase acidic protein	polymer	197	23134.3	1	UniProt (A0A4P2TE19) UniProt (C6H0Y9) Pfam (PF00603)	Influenza A virus
2	B (B)	DNA (5'-D(P*TP*AP*GP*CP*AP*T)-3')	polymer	8	2416.6	1		synthetic construct
3	C, D (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	E (A)	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)
5	F (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
6	G, H (A, B)	water	water		18.0	111	Chemie (HOH)

Sequence modifications

A: 1 - 50 (UniProt: A0A4P2TE19)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gly 51	-	linker
Gly 52	-	linker
Ser 53	-	linker

A: 73 - 196 (UniProt: C6H0Y9)

PDB	External Database	Details
Asp 119	Glu 119	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	25550.9
Non-Polymers*	Number of molecules	4
	Total formula weight	335.2
All*	Total formula weight	25886.1

*Water molecules are not included.