6W5M
Cryo-EM structure of MLL1 in complex with RbBP5, WDR5, SET1, and ASH2L bound to the nucleosome (Class02)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Retinoblastoma-binding protein 5 | polymer | 538 | 59179.4 | 1 | UniProt (Q15291) Pfam (PF00400) | Homo sapiens (Human) | RBBP-5,Retinoblastoma-binding protein RBQ-3 |
| 2 | B (B) | WD repeat-containing protein 5 | polymer | 313 | 34391.0 | 1 | UniProt (P61964) Pfam (PF00400) | Homo sapiens (Human) | BMP2-induced 3-kb gene protein |
| 3 | C (C) | Histone-lysine N-methyltransferase 2A | polymer | 209 | 24141.7 | 1 | UniProt (Q03164) Pfam (PF00856) | Homo sapiens (Human) | Lysine N-methyltransferase 2A,ALL-1,CXXC-type zinc finger protein 7,Myeloid/lymphoid or mixed-lineage leukemia,Myeloid/lymphoid or mixed-lineage leukemia protein 1,Trithorax-like protein,Zinc finger protein HRX |
| 4 | D (D) | Set1/Ash2 histone methyltransferase complex subunit ASH2 | polymer | 534 | 60244.6 | 1 | UniProt (Q9UBL3) Pfam (PF21257) Pfam (PF21198) Pfam (PF00622) | Homo sapiens (Human) | ASH2-like protein |
| 5 | E, I (G, K) | Histone H3.2 | polymer | 136 | 15435.1 | 2 | UniProt (P84233) Pfam (PF00125) | Xenopus laevis (African clawed frog) | Histone H3 |
| 6 | F, J (H, L) | Histone H4 | polymer | 103 | 11394.4 | 2 | UniProt (P62799) Pfam (PF15511) | Xenopus laevis (African clawed frog) | |
| 7 | G, K (I, M) | Histone H2A type 1 | polymer | 129 | 13978.2 | 2 | UniProt (P06897) Pfam (PF00125) Pfam (PF16211) | Xenopus laevis (African clawed frog) | |
| 8 | H, L (J, N) | Histone H2B 1.1 | polymer | 123 | 13655.9 | 2 | UniProt (P02281) Pfam (PF00125) | Xenopus laevis (African clawed frog) | H2B1.1 |
| 9 | M (O) | DNA (147-MER) | polymer | 147 | 45138.8 | 1 | synthetic construct | ||
| 10 | N (P) | DNA (147-MER) | polymer | 147 | 45610.0 | 1 | synthetic construct |
Sequence modifications
A: 2 - 538 (UniProt: Q15291)
C: 3762 - 3969 (UniProt: Q03164)
D: 2 - 534 (UniProt: Q9UBL3)
G, K: 0 - 135 (UniProt: P84233)
I, M: 1 - 129 (UniProt: P06897)
J, N: 1 - 122 (UniProt: P02281)
| PDB | External Database | Details |
|---|---|---|
| Ser 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 3761 | - | expression tag |
| Ile 3861 | Asn 3861 | conflict |
| Leu 3867 | Gln 3867 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ser 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 102 | Gly 103 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Arg 99 | Gly 100 | engineered mutation |
| Ser 123 | Ala 124 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Thr 29 | Ser 33 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 377633.0 | |
| All* | Total formula weight | 377633.0 |
