6W4T
APE1 Y269A phosphorothioate substrate complex with abasic DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, B) | DNA-(apurinic or apyrimidinic site) lyase | polymer | 276 | 31064.4 | 2 | UniProt (P27695) Pfam (PF03372) | Homo sapiens (Human) | APEX nuclease,APEN,Apurinic-apyrimidinic endonuclease 1,APE-1,REF-1,Redox factor-1 |
| 2 | B, E (P, C) | DNA (5'-D(P*GP*CP*TP*GP*AP*TP*GP*CP*GP*TP*(48Z)P*CP*GP*AP*CP*GP*GP*AP*TP*CP*C)-3') | polymer | 21 | 6347.1 | 2 | synthetic construct | ||
| 3 | C, F (V, F) | DNA (5'-D(*GP*GP*AP*TP*CP*CP*GP*TP*CP*GP*GP*AP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3') | polymer | 21 | 6449.2 | 2 | synthetic construct | ||
| 4 | G, H, I, J (A, P, V, B) | water | water | 18.0 | 39 | Chemie (HOH) |
Sequence modifications
A, B: 43 - 318 (UniProt: P27695)
| PDB | External Database | Details |
|---|---|---|
| Ala 138 | Cys 138 | engineered mutation |
| Ala 269 | Tyr 269 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 87721.3 | |
| All* | Total formula weight | 87721.3 |
