6W4H

1.80 Angstrom Resolution Crystal Structure of NSP16 - NSP10 Complex from SARS-CoV-2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	2'-O-methyltransferase	polymer	301	33627.5	1	UniProt (P0DTD1) Pfam (PF06460)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	Non-structural protein 16,nsp16,SARS-CoV-2 NSP16
2	B (B)	Non-structural protein 10	polymer	142	15075.2	1	UniProt (P0DTD1) Pfam (PF09401)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	nsp10,Growth factor-like peptide,GFL,SARS-CoV-2 NSP10
3	C (A)	SULFITE ION	non-polymer		80.1	1	Chemie (SO3)
4	D (A)	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
5	E, F (A)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
6	G, J (A, B)	beta-D-fructopyranose	non-polymer		180.2	2	Chemie (BDF)
7	H, I (B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
8	K, L (A, B)	water	water		18.0	442	Chemie (HOH)

Sequence modifications

A: 6799 - 7096 (UniProt: P0DTD1)

PDB	External Database	Details
Ser 6796	-	expression tag
Asn 6797	-	expression tag
Ala 6798	-	expression tag

B: 4254 - 4392 (UniProt: P0DTD1)

PDB	External Database	Details
Ser 4251	-	expression tag
Asn 4252	-	expression tag
Ala 4253	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	48702.7
Non-Polymers*	Number of molecules	8
	Total formula weight	1087.7
All*	Total formula weight	49790.4

*Water molecules are not included.