6VZG

Cryo-EM structure of Sth1-Arp7-Arp9-Rtt102

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (L)	Actin-related protein 7	polymer	477	53863.0	1	UniProt (Q12406) Pfam (PF00022)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Actin-like protein ARP7,Chromatin structure-remodeling complex protein ARP7,SWI/SNF complex component ARP7
2	B (M)	Actin-like protein ARP9	polymer	467	53131.9	1	UniProt (Q05123) Pfam (PF00022)	Saccharomyces cerevisiae (Baker's yeast)	Chromatin structure-remodeling complex protein ARP9,SWI/SNF complex component ARP9
3	C (K)	Nuclear protein STH1/NPS1	polymer	813	95077.3	1	UniProt (P32597) Pfam (PF07529) Pfam (PF00176) Pfam (PF00271) Pfam (PF14619)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	ATP-dependent helicase STH1,Chromatin structure-remodeling complex protein STH1,SNF2 homolog
4	D (N)	Regulator of Ty1 transposition protein 102	polymer	157	17817.6	1	UniProt (P53330) Pfam (PF09510)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
5	E (L)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)

Sequence modifications

K: 301 - 1097 (UniProt: P32597)

PDB	External Database	Details
Met 285	-	initiating methionine
Gly 286	-	expression tag
Ser 287	-	expression tag
Ser 288	-	expression tag
His 289	-	expression tag
His 290	-	expression tag
His 291	-	expression tag
His 292	-	expression tag
His 293	-	expression tag
His 294	-	expression tag
Ser 295	-	expression tag
Gln 296	-	expression tag
Asp 297	-	expression tag
Pro 298	-	expression tag
Asn 299	-	expression tag
Ser 300	-	expression tag
Lys 372	Arg 372	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	219889.8
Non-Polymers*	Number of molecules	1
	Total formula weight	507.2
All*	Total formula weight	220397.0

*Water molecules are not included.