6VYP

Crystal structure of the LSD1/CoREST histone demethylase bound to its nucleosome substrate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, a, e	Histone H3	polymer	135	15305.9	4	UniProt (A0A310TTQ1) Pfam (PF00125) UniProt (by SIFTS) (P84233) In PDB	Xenopus laevis (African clawed frog)
2	B, F, b, f	Histone H4	polymer	102	11263.2	4	UniProt (P62799) Pfam (PF15511) In PDB	Xenopus laevis (African clawed frog)
3	C, G, c, g	Histone H2A type 1	polymer	129	13978.2	4	UniProt (P06897) Pfam (PF00125) Pfam (PF16211) In PDB	Xenopus laevis (African clawed frog)
4	D, H, d, h	Histone H2B 1.1	polymer	122	13524.8	4	UniProt (P02281) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)	H2B1.1
5	I, i	DNA (191-MER)	polymer	191	58694.4	2		synthetic construct
6	J, j	DNA (191-MER)	polymer	191	59245.7	2		synthetic construct
7	M, m, K, k	Lysine-specific histone demethylase 1A	polymer	684	75732.6	4	UniProt (O60341) Pfam (PF04433) Pfam (PF01593) In PDB	Homo sapiens (Human)	BRAF35-HDAC complex protein BHC110,Flavin-containing amine oxidase domain-containing protein 2
8	N, n, L, l	REST corepressor 1	polymer	157	17956.4	4	UniProt (Q9UKL0) Pfam (PF20878) Pfam (PF00249) In PDB	Homo sapiens (Human)	Protein CoREST
9	M, m, K, k	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	4	Chemie (FAD)

Sequence modifications

A, E, a, e: 1 - 135 (UniProt: A0A310TTQ1)

PDB	External Database	Details
Met 4	Lys 5	engineered mutation

C, G, c, g: 1 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	engineered mutation
Ser 123	Ala 124	engineered mutation

D, H, d, h: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Thr 29	Ser 33	engineered mutation

M, m, K, k: 171 - 852 (UniProt: O60341)

N, n, L, l: 286 - 440 (UniProt: Q9UKL0)

PDB	External Database	Details
Gly 284	-	expression tag
Ser 285	-	expression tag

Sequence viewer

Contents of the asymmetric unit

*Water molecules are not included.