6VYP
Crystal structure of the LSD1/CoREST histone demethylase bound to its nucleosome substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.771, 335.770, 174.633 |
| Unit cell angles | 90.00, 91.07, 90.00 |
Refinement procedure
| Resolution | 20.170 - 4.990 |
| R-factor | 0.2309 |
| Rwork | 0.229 |
| R-free | 0.27740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lz0 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.468 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (0.5.23) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.170 | 20.170 | 5.150 |
| High resolution limit [Å] | 4.990 | 19.960 | 4.990 |
| Rmerge | 0.079 | 0.032 | 1.340 |
| Rmeas | 0.093 | 0.038 | 1.575 |
| Rpim | 0.050 | 0.021 | 0.822 |
| Total number of observations | 175610 | 74 | 17137 |
| Number of reflections | 50839 | 26 | 4742 |
| <I/σ(I)> | 8.1 | 27.7 | 0.9 |
| Completeness [%] | 98.2 | 3.1 | 99.9 |
| Redundancy | 3.5 | 2.8 | 3.6 |
| CC(1/2) | 0.998 | 0.998 | 0.352 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 277 | 25 mM HEPES pH7.5, 75 mM triammonium citrate, 10% PEG2000-MME, Modified Microbatch under oil |
