6VUG

Diabody bound to a Reverse Transcriptase Aptamer Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Reverse transcriptase/ribonuclease H	polymer	555	63875.1	1	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075)	Human immunodeficiency virus type 1 (HIV-1)	Gag-Pol polyprotein, Pr160Gag-Pol
2	B (B)	REVERSE TRANSCRIPTASE P51	polymer	444	51928.6	1	UniProt (A0A076Q3N8) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) UniProt (by SIFTS) (P03366)	Human immunodeficiency virus 1 (HIV-1)
3	C (C)	Light chain variable fragment	polymer	120	13249.6	1		Homo sapiens (Human)
4	D (D)	Heavy chain variable fragment	polymer	124	13185.6	1		Homo sapiens (Human)
5	E (E)	DNA (38-MER)	polymer	38	11748.5	1		Human immunodeficiency virus 1 (HIV-1)
6	F, G, H, I, J... (B, C)	SULFATE ION	non-polymer		96.1	11	Chemie (SO4)
7	N (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)

Sequence modifications

A: 1 - 555 (UniProt: P03366)

PDB	External Database	Details
Ser 280	Cys 879	conflict

B: 1 - 428 (UniProt: A0A076Q3N8)

PDB	External Database	Details
Met -15	-	initiating methionine
Ala -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Ala -7	-	expression tag
Leu -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Leu -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Lys 172	Arg 339	engineered mutation
Ser 280	Cys 447	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	153987.5
Non-Polymers*	Number of molecules	12
	Total formula weight	1148.8
All*	Total formula weight	155136.3

*Water molecules are not included.