6VPP

Cryo-EM structure of microtubule-bound KLP61F motor with tail domain in the nucleotide-free state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Tubulin alpha-1A chain	polymer	451	50121.3	1	UniProt (P02550) Pfam (PF00091) Pfam (PF03953) In PDB	Sus scrofa (Pig)	Alpha-tubulin 1,Tubulin alpha-1 chain
2	B	Tubulin beta chain	polymer	445	49907.8	1	UniProt (P02554) Pfam (PF00091) Pfam (PF03953) In PDB	Sus scrofa (Pig)	Beta-tubulin
3	C	Kinesin-like protein Klp61F	polymer	377	42627.3	1	UniProt (P46863) Pfam (PF00225) In PDB	Drosophila melanogaster (Fruit fly)	Bipolar kinesin KRP-130
4	A	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
5	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	B	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)

Sequence modifications

C: 1 - 369 (UniProt: P46863)

PDB	External Database	Details
Leu 370	-	expression tag
Glu 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag
His 375	-	expression tag
His 376	-	expression tag
His 377	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	142656.4
Non-Polymers*	Number of molecules	3
	Total formula weight	990.7
All*	Total formula weight	143647.1

*Water molecules are not included.