6VPC
Structure of the SpCas9 DNA adenine base editor - ABE8e
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Cas9 (SpCas9) single-guide RNA (sgRNA) | polymer | 82 | 26357.6 | 1 | Streptococcus pyogenes | ||
| 2 | B (B) | CRISPR-associated endonuclease Cas9 | polymer | 1361 | 157871.0 | 1 | UniProt (A0A4U7H5M9) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) UniProt (by SIFTS) (Q99ZW2) | Streptococcus pyogenes | |
| 3 | C (C) | DNA target strand (TS) | polymer | 50 | 15390.9 | 1 | synthetic construct | ||
| 4 | D (D) | DNA non-target strand (NTS) | polymer | 50 | 15436.8 | 1 | synthetic construct | ||
| 5 | E, F (E, F) | t-RNA adenine deaminase A v8e (TadA-8e) | polymer | 227 | 24657.9 | 2 | Pfam (PF00383) UniProt (by SIFTS) (P68398) | Escherichia coli | |
| 6 | G, H, I (A) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 7 | J, K (E, F) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
B: 201 - 1561 (UniProt: A0A4U7H5M9)
| PDB | External Database | Details |
|---|---|---|
| Ala 207 | Asp 10 | conflict |
| Glu 221 | Asp 24 | conflict |
| Ala 1037 | His 840 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 264372.1 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 203.7 | |
| All* | Total formula weight | 264575.8 |
