6VPC
Structure of the SpCas9 DNA adenine base editor - ABE8e
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| B | 0003677 | molecular_function | DNA binding |
| B | 0003723 | molecular_function | RNA binding |
| B | 0004518 | molecular_function | nuclease activity |
| B | 0004519 | molecular_function | endonuclease activity |
| B | 0004520 | molecular_function | DNA endonuclease activity |
| B | 0004527 | molecular_function | exonuclease activity |
| B | 0008408 | molecular_function | 3'-5' exonuclease activity |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0043571 | biological_process | maintenance of CRISPR repeat elements |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0051607 | biological_process | defense response to virus |
| E | 0002100 | biological_process | tRNA wobble adenosine to inosine editing |
| E | 0003824 | molecular_function | catalytic activity |
| E | 0006382 | biological_process | adenosine to inosine editing |
| E | 0008033 | biological_process | tRNA processing |
| E | 0008251 | molecular_function | tRNA-specific adenosine deaminase activity |
| E | 0008270 | molecular_function | zinc ion binding |
| E | 0016787 | molecular_function | hydrolase activity |
| E | 0042803 | molecular_function | protein homodimerization activity |
| E | 0046872 | molecular_function | metal ion binding |
| E | 0052717 | molecular_function | tRNA-specific adenosine-34 deaminase activity |
| F | 0002100 | biological_process | tRNA wobble adenosine to inosine editing |
| F | 0003824 | molecular_function | catalytic activity |
| F | 0006382 | biological_process | adenosine to inosine editing |
| F | 0008033 | biological_process | tRNA processing |
| F | 0008251 | molecular_function | tRNA-specific adenosine deaminase activity |
| F | 0008270 | molecular_function | zinc ion binding |
| F | 0016787 | molecular_function | hydrolase activity |
| F | 0042803 | molecular_function | protein homodimerization activity |
| F | 0046872 | molecular_function | metal ion binding |
| F | 0052717 | molecular_function | tRNA-specific adenosine-34 deaminase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 1 |
| Details | binding site for residue MG A 102 |
| Chain | Residue |
| A | U51 |
| site_id | AC2 |
| Number of Residues | 2 |
| Details | binding site for residue MG A 103 |
| Chain | Residue |
| A | A66 |
| B | THR1299 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | binding site for residue ZN E 201 |
| Chain | Residue |
| E | HIS57 |
| E | CYS87 |
| E | CYS90 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | binding site for residue ZN F 201 |
| Chain | Residue |
| F | CYS90 |
| D | 8AZ26 |
| F | HIS57 |
| F | CYS87 |
Functional Information from PROSITE/UniProt
| site_id | PS00903 |
| Number of Residues | 38 |
| Details | CYT_DCMP_DEAMINASES_1 Cytidine and deoxycytidylate deaminases zinc-binding region signature. HAEimALrqgglvmqnyrlidatlyvtfe...........PCvm......CagaM |
| Chain | Residue | Details |
| E | HIS57-MET94 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 17 |
| Details | Region: {"description":"ARM","evidences":[{"source":"PubMed","id":"24505130","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 177 |
| Details | Region: {"description":"RuvC-III","evidences":[{"source":"PubMed","id":"24505130","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Motif: {"description":"PAM substrate-binding","evidences":[{"source":"PubMed","id":"25079318","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | Active site: {"description":"For RuvC-like nuclease domain","evidences":[{"source":"PubMed","id":"24529477","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Proton acceptor for HNH nuclease domain","evidences":[{"source":"PubMed","id":"24529477","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 6 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"24505130","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 222 |
| Details | Domain: {"description":"CMP/dCMP-type deaminase","evidences":[{"source":"PROSITE-ProRule","id":"PRU01083","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"16700551","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 6 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00972","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"16700551","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
