6VN7
Cryo-EM structure of an activated VIP1 receptor-G protein complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Vasoactive intestinal polypeptide receptor 1 | polymer | 582 | 66245.2 | 1 | Pfam (PF02793) Pfam (PF00002) UniProt (by SIFTS) (P32241) | Homo sapiens (Human) | |
| 2 | B (L) | Pituitary adenylate cyclase-activating polypeptide | polymer | 27 | 3154.6 | 1 | UniProt (P18509) Pfam (PF00123) | Homo sapiens | PACAP |
| 3 | C (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45727.4 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (Human) | Adenylate cyclase-stimulating G alpha protein |
| 4 | D (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 377 | 41055.9 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (Human) | Transducin beta chain 1 |
| 5 | E (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (Human) | G gamma-I |
| 6 | F (N) | Nanobody 35 | polymer | 134 | 14714.3 | 1 | synthetic construct | ||
| 7 | G, H, I, J, K... (R) | CHOLESTEROL | non-polymer | 386.7 | 6 | Chemie (CLR) | |||
| 8 | M, N, O, P (R) | PALMITIC ACID | non-polymer | 256.4 | 4 | Chemie (PLM) |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Asn 54 | Ser 54 | conflict |
| Asp 188 | Ala 188 | conflict |
| Ala 226 | Gly 226 | conflict |
| Ala 268 | Glu 268 | conflict |
| Lys 271 | Asn 271 | conflict |
| Asp 274 | Lys 274 | conflict |
| Lys 280 | Arg 280 | conflict |
| Asp 284 | Thr 284 | conflict |
| Thr 285 | Ile 285 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met -10 | - | initiating methionine |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| Gly 341 | - | expression tag |
| Ser 342 | - | expression tag |
| Ser 343 | - | expression tag |
| Gly 344 | - | expression tag |
| Gly 345 | - | expression tag |
| Gly 346 | - | expression tag |
| Gly 347 | - | expression tag |
| Ser 348 | - | expression tag |
| Gly 349 | - | expression tag |
| Gly 350 | - | expression tag |
| Gly 351 | - | expression tag |
| Gly 352 | - | expression tag |
| Ser 353 | - | expression tag |
| Ser 354 | - | expression tag |
| Gly 355 | - | expression tag |
| Val 356 | - | expression tag |
| Ser 357 | - | expression tag |
| Gly 358 | - | expression tag |
| Trp 359 | - | expression tag |
| Arg 360 | - | expression tag |
| Leu 361 | - | expression tag |
| Phe 362 | - | expression tag |
| Lys 363 | - | expression tag |
| Lys 364 | - | expression tag |
| Ile 365 | - | expression tag |
| Ser 366 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 178758.6 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 3345.6 | |
| All* | Total formula weight | 182104.2 |
