6VN7
Cryo-EM structure of an activated VIP1 receptor-G protein complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | R | Vasoactive intestinal polypeptide receptor 1 | polymer | 582 | 66245.2 | 1 | Pfam (PF02793) Pfam (PF00002) UniProt (by SIFTS) (P32241) In PDB | Homo sapiens (Human) | |
2 | L | Pituitary adenylate cyclase-activating polypeptide | polymer | 27 | 3154.6 | 1 | UniProt (P18509) Pfam (PF00123) In PDB | Homo sapiens | PACAP |
3 | A | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45727.4 | 1 | UniProt (P63092) Pfam (PF00503) In PDB | Homo sapiens (Human) | Adenylate cyclase-stimulating G alpha protein |
4 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 377 | 41055.9 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (Human) | Transducin beta chain 1 |
5 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (Human) | G gamma-I |
6 | N | Nanobody 35 | polymer | 134 | 14714.3 | 1 | synthetic construct | ||
7 | R | CHOLESTEROL | non-polymer | 386.7 | 6 | Chemie (CLR) | |||
8 | R | PALMITIC ACID | non-polymer | 256.4 | 4 | Chemie (PLM) |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
PDB | External Database | Details |
---|---|---|
Asn 54 | Ser 54 | conflict |
Asp 188 | Ala 188 | conflict |
Ala 226 | Gly 226 | conflict |
Ala 268 | Glu 268 | conflict |
Lys 271 | Asn 271 | conflict |
Asp 274 | Lys 274 | conflict |
Lys 280 | Arg 280 | conflict |
Asp 284 | Thr 284 | conflict |
Thr 285 | Ile 285 | conflict |
PDB | External Database | Details |
---|---|---|
Met -10 | - | initiating methionine |
His -9 | - | expression tag |
His -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
Leu -1 | - | expression tag |
Leu 0 | - | expression tag |
Gln 1 | - | expression tag |
Gly 341 | - | expression tag |
Ser 342 | - | expression tag |
Ser 343 | - | expression tag |
Gly 344 | - | expression tag |
Gly 345 | - | expression tag |
Gly 346 | - | expression tag |
Gly 347 | - | expression tag |
Ser 348 | - | expression tag |
Gly 349 | - | expression tag |
Gly 350 | - | expression tag |
Gly 351 | - | expression tag |
Gly 352 | - | expression tag |
Ser 353 | - | expression tag |
Ser 354 | - | expression tag |
Gly 355 | - | expression tag |
Val 356 | - | expression tag |
Ser 357 | - | expression tag |
Gly 358 | - | expression tag |
Trp 359 | - | expression tag |
Arg 360 | - | expression tag |
Leu 361 | - | expression tag |
Phe 362 | - | expression tag |
Lys 363 | - | expression tag |
Lys 364 | - | expression tag |
Ile 365 | - | expression tag |
Ser 366 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 178758.6 | |
Non-Polymers* | Number of molecules | 10 |
Total formula weight | 3345.6 | |
All* | Total formula weight | 182104.2 |