6VMS

Structure of a D2 dopamine receptor-G-protein complex in a lipid membrane

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40382.0	1	UniProt (P10824) Pfam (PF00503) In PDB	Rattus norvegicus (Rat)	Adenylate cyclase-inhibiting G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	82	9137.5	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
4	E	scFv16	polymer	259	27784.9	1		Homo sapiens (Human)
5	R	Endolysin,D(2) dopamine receptor,D(2) dopamine receptor	polymer	450	51384.1	1	UniProt (D9IEF7) UniProt (P14416) Pfam (PF00959) Pfam (PF00001) In PDB	Enterobacteria phage T4	Lysis protein,Lysozyme,Muramidase,Dopamine D2 receptor,Dopamine D2 receptor
6	R	bromoergocryptine	non-polymer		654.6	1	Chemie (08Y)

Sequence modifications

A: 1 - 354 (UniProt: P10824)

PDB	External Database	Details
Asn 47	Ser 47	conflict
Ala 203	Gly 203	conflict
Ala 245	Glu 245	conflict

C: 2 - 71 (UniProt: P59768)

PDB	External Database	Details
Met -10	-	expression tag
Gly -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Gly 1	-	expression tag

R: -132 - 27 (UniProt: D9IEF7)

PDB	External Database	Details
Asp -140	-	expression tag
Tyr -139	-	expression tag
Lys -138	-	expression tag
Asp -137	-	expression tag
Asp -136	-	expression tag
Asp -135	-	expression tag
Asp -134	-	expression tag
Ala -133	-	expression tag
Asn -114	Asp 20	conflict
Thr -80	Cys 54	conflict
Ala -37	Cys 97	conflict
Ala 28	-	linker

R: 29 - 226 (UniProt: P14416)

PDB	External Database	Details
Ile 205	Thr 205	conflict
Leu 222	Arg 222	conflict
Asn 226	Lys 226	conflict

R: 361 - 443 (UniProt: P14416)

PDB	External Database	Details
Leu 374	Met 374	conflict
Tyr 378	Val 378	conflict
Leu 381	Val 381	conflict
Ile 421	Val 421	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	166105.4
Non-Polymers*	Number of molecules	1
	Total formula weight	654.6
All*	Total formula weight	166760.0

*Water molecules are not included.