6VHY
NpsA-ThdA, an artificially fused Adenylation-PCP di-domain NRPS from Klebsiella oxytoca
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | NpsA Adenylation Domain, Non-ribosomal peptide synthetase fusion protein | polymer | 586 | 64062.5 | 4 | UniProt (A0A2U4DY99) UniProt (A0A2U4DNX7) | Klebsiella oxytoca | |
| 2 | E, F, G, H (A, B, C, D) | 5'-deoxy-5'-({[(2R)-2-{[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl]sulfanyl}-2-(3-hydroxyphenyl)ethyl]sulfonyl}amino)adenosine | non-polymer | 806.8 | 4 | Chemie (QXD) | |||
| 3 | I (C) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 506 (UniProt: A0A2U4DY99)
A, B, C, D: 509 - 584 (UniProt: A0A2U4DNX7)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| His 0 | - | expression tag |
| Ser 258 | Leu 258 | variant |
| Ala 312 | Glu 312 | engineered mutation |
| Ala 313 | Glu 313 | engineered mutation |
| Ala 314 | Gln 314 | engineered mutation |
| Ala 507 | - | linker |
| Ser 508 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 256250.1 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 3227.2 | |
| All* | Total formula weight | 259477.3 |
