6VHQ

Crystal structure of Bacillus subtilis levansucrase (D86A/E342A) in complex with oligosaccharides

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Glycoside hydrolase family 68 protein	polymer	466	52360.8	2	UniProt (A0PFL2) Pfam (PF02435) UniProt (by SIFTS) (P05655)	Bacillus subtilis	Levansucrase
2	C, D, E (C, D, E)	beta-D-fructofuranose-(2-6)-beta-D-fructofuranose-(2-6)-beta-D-fructofuranose-(2-6)-beta-D-fructofuranose-(2-6)-beta-D-fructofuranose-(2-6)-beta-D-fructofuranose	branched		990.9	3	In PDB GlyTouCan (G00825VL)
3	F (F)	beta-D-fructofuranose-(2-6)-beta-D-fructofuranose	branched		342.3	1	In PDB BIRD (PRD_900051) GlyTouCan (G51829YA)		levanbiose
4	G, N (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	H, I, J, K, L... (A, B)	BROMIDE ION	non-polymer		79.9	9	Chemie (BR)
6	R, S (A, B)	water	water		18.0	540	Chemie (HOH)

Sequence modifications

A, B: 30 - 473 (UniProt: A0PFL2)

PDB	External Database	Details
Ala 86	Asp 86	engineered mutation
Ala 342	Glu 342	engineered mutation
Thr 474	-	expression tag
Asp 475	-	expression tag
Pro 476	-	expression tag
Asn 477	-	expression tag
Ser 478	-	expression tag
Ser 479	-	expression tag
Ser 480	-	expression tag
Val 481	-	expression tag
Asp 482	-	expression tag
Lys 483	-	expression tag
Leu 484	-	expression tag
Ala 485	-	expression tag
Ala 486	-	expression tag
Ala 487	-	expression tag
Leu 488	-	expression tag
Glu 489	-	expression tag
His 490	-	expression tag
His 491	-	expression tag
His 492	-	expression tag
His 493	-	expression tag
His 494	-	expression tag
His 495	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	104721.6
Branched	Number of molecules	4
	Total formula weight	3314.9
Non-Polymers*	Number of molecules	11
	Total formula weight	799.3
All*	Total formula weight	108835.8

*Water molecules are not included.