6VHQ
Crystal structure of Bacillus subtilis levansucrase (D86A/E342A) in complex with oligosaccharides
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005576 | cellular_component | extracellular region |
| A | 0009758 | biological_process | carbohydrate utilization |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016757 | molecular_function | glycosyltransferase activity |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0050053 | molecular_function | levansucrase activity |
| B | 0005576 | cellular_component | extracellular region |
| B | 0009758 | biological_process | carbohydrate utilization |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016757 | molecular_function | glycosyltransferase activity |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0050053 | molecular_function | levansucrase activity |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"14517548","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Proton donor/acceptor","evidences":[{"source":"PubMed","id":"14517548","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 14 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"14517548","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1PT2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3BYN","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 10 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"14517548","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18596022","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32553967","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"33303628","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1OYG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1PT2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2VDT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3BYJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3BYK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3BYN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6PWQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6VHQ","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Site: {"description":"Transition state stabilizer","evidences":[{"source":"PubMed","id":"14517548","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 875 |
| Chain | Residue | Details |
| A | ALA86 | covalent catalysis |
| A | SER164 | steric locator |
| A | ASP247 | electrostatic stabiliser |
| A | ALA342 | proton shuttle (general acid/base) |
| site_id | MCSA2 |
| Number of Residues | 4 |
| Details | M-CSA 875 |
| Chain | Residue | Details |
| B | ALA86 | covalent catalysis |
| B | SER164 | steric locator |
| B | ASP247 | electrostatic stabiliser |
| B | ALA342 | proton shuttle (general acid/base) |
