6VEN

Yeast COMPASS in complex with a ubiquitinated nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Histone H3.2	polymer	135	15251.8	2	UniProt (P84233) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)
2	B, F	Histone H4	polymer	102	11263.2	2	UniProt (P62799) Pfam (PF15511) In PDB	Xenopus laevis (African clawed frog)
3	C, G	Histone H2A type 1	polymer	129	13978.2	2	UniProt (P06897) Pfam (PF00125) Pfam (PF16211) In PDB	Xenopus laevis (African clawed frog)
4	D, H	Histone H2B 1.1	polymer	122	13498.7	2	UniProt (P02281) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)	H2B1.1
5	I	601 DNA (146-MER)	polymer	146	44825.6	1		synthetic construct
6	J	601 DNA (146-MER)	polymer	146	45305.9	1		synthetic construct
7	K	Ubiquitin	polymer	80	9036.4	1	UniProt (P0CG48) Pfam (PF00240) In PDB	Homo sapiens (Human)
8	L	COMPASS component SWD3	polymer	315	34786.6	1	UniProt (P38123) Pfam (PF00400) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Complex proteins associated with SET1 protein SWD3,Set1C component SWD3
9	M	COMPASS component SWD1	polymer	426	48702.6	1	UniProt (P39706) Pfam (PF00400) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Complex proteins associated with SET1 protein SWD1,Set1C component SWD1
10	N	Histone-lysine N-methyltransferase, H3 lysine-4 specific	polymer	358	41508.5	1	UniProt (P38827) Pfam (PF11764) Pfam (PF00856) In PDB	Saccharomyces cerevisiae (Baker's yeast)	COMPASS component SET1,Lysine N-methyltransferase 2,SET domain-containing protein 1
11	O	COMPASS component BRE2	polymer	505	58425.3	1	UniProt (P43132) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Brefeldin-A sensitivity protein 2,Complex proteins associated with SET1 protein BRE2,Set1C component BRE2
12	P, Q	COMPASS component SDC1	polymer	175	19466.2	2	UniProt (Q03323) Pfam (PF05186) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Complex proteins associated with SET1 protein SDC1,Set1C component SDC1,Suppressor of CDC25 protein 1
13	R	COMPASS component SPP1	polymer	391	45489.5	1	UniProt (Q03012) Pfam (PF00628) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Complex proteins associated with SET1 protein SPP1,Set1C component SPP1,Suppressor of PRP protein 1
14	N	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
15	N	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)

Sequence modifications

A, E: 1 - 135 (UniProt: P84233)

PDB	External Database	Details
Nle 4	Lys 5	engineered mutation
Nle 90	Met 91	engineered mutation
Ala 102	Gly 103	engineered mutation
Nle 120	Met 121	engineered mutation

C, G: 1 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	engineered mutation
Ser 123	Ala 124	engineered mutation

D, H: 4 - 125 (UniProt: P02281)

PDB	External Database	Details
Thr 32	Ser 33	engineered mutation
Cys 120	Lys 121	engineered mutation

K: 1 - 76 (UniProt: P0CG48)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Cys 76	Gly 76	engineered mutation

N: 762 - 1080 (UniProt: P38827)

PDB	External Database	Details
Met 723	-	expression tag
His 724	-	expression tag
His 725	-	expression tag
His 726	-	expression tag
His 727	-	expression tag
His 728	-	expression tag
His 729	-	expression tag
Gly 730	-	expression tag
Ser 731	-	expression tag
Ser 732	-	expression tag
Asp 733	-	expression tag
Tyr 734	-	expression tag
Lys 735	-	expression tag
Asp 736	-	expression tag
His 737	-	expression tag
Asp 738	-	expression tag
Gly 739	-	expression tag
Asp 740	-	expression tag
Tyr 741	-	expression tag
Lys 742	-	expression tag
Asp 743	-	expression tag
His 744	-	expression tag
Asp 745	-	expression tag
Ile 746	-	expression tag
Asp 747	-	expression tag
Tyr 748	-	expression tag
Lys 749	-	expression tag
Asp 750	-	expression tag
Asp 751	-	expression tag
Asp 752	-	expression tag
Asp 753	-	expression tag
Lys 754	-	expression tag
Glu 755	-	expression tag
Asn 756	-	expression tag
Leu 757	-	expression tag
Tyr 758	-	expression tag
Phe 759	-	expression tag
Gln 760	-	expression tag
Gly 761	-	expression tag

R: 2 - 353 (UniProt: Q03012)

PDB	External Database	Details
Met -37	-	expression tag
Trp -36	-	expression tag
Ser -35	-	expression tag
His -34	-	expression tag
Pro -33	-	expression tag
Gln -32	-	expression tag
Phe -31	-	expression tag
Glu -30	-	expression tag
Lys -29	-	expression tag
Gly -28	-	expression tag
Gly -27	-	expression tag
Gly -26	-	expression tag
Ser -25	-	expression tag
Gly -24	-	expression tag
Gly -23	-	expression tag
Gly -22	-	expression tag
Ser -21	-	expression tag
Gly -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
Ala -16	-	expression tag
Trp -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
Pro -12	-	expression tag
Gln -11	-	expression tag
Phe -10	-	expression tag
Glu -9	-	expression tag
Lys -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	18
	Total formula weight	474996.7
Non-Polymers*	Number of molecules	2
	Total formula weight	463.8
All*	Total formula weight	475460.6

*Water molecules are not included.