6VCM

Crystal structure of E.coli RppH-DapF in complex with GTP, Mg2+ and F-

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Diaminopimelate epimerase	polymer	275	30154.4	1	UniProt (P0A6K1) Pfam (PF01678)	Escherichia coli (strain K12)	DAP epimerase,PLP-independent amino acid racemase
2	B (B)	RNA pyrophosphohydrolase	polymer	161	18965.8	1	UniProt (P0A776) Pfam (PF00293)	Escherichia coli (strain K12)	(Di)nucleoside polyphosphate hydrolase,Ap5A pyrophosphatase
3	C (B)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL) PubChem (312)
4	D, E, F, G (B)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG) PubChem (888)
5	H (B)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466)
6	I (B)	FLUORIDE ION	non-polymer		19.0	1	Chemie (F) PubChem (28179)
7	J, K (A, B)	water	water		18.0	73	Chemie (HOH)

Sequence modifications

A: 1 - 274 (UniProt: P0A6K1)

PDB	External Database	Details
Ser 0	-	expression tag
Ala 36	Arg 36	engineered mutation
Ala 268	Tyr 268	engineered mutation

B: 1 - 160 (UniProt: P0A776)

PDB	External Database	Details
Ser 0	-	expression tag
Ala 159	Gln 159	engineered mutation
Ala 160	Glu 160	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	49120.2
Non-Polymers*	Number of molecules	7
	Total formula weight	674.9
All*	Total formula weight	49795.0

*Water molecules are not included.