6VCM

Crystal structure of E.coli RppH-DapF in complex with GTP, Mg2+ and F-

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	C 2 2 21
Unit cell lengths	162.357, 192.948, 51.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.900 - 2.350
R-factor	0.2138
Rwork	0.212
R-free	0.23450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6d1v
RMSD bond length	0.003
RMSD bond angle	0.690
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3546)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.400
High resolution limit [Å]	2.350	2.350
Number of reflections	33797	2208
<I/σ(I)>	22.2
Completeness [%]	99.9
Redundancy	6.6
CC(1/2)	0.994	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.2	291.15	30% (v/v) PEG400, 0.1 M CHES, pH 9.2