6V9K

CRYSTAL STRUCTURE OF THE HYBRID C-TERMINAL DOMAIN OF ENZYME I OF THE BACTERIAL PHOSPHOTRANSFERASE SYSTEM FORMED BY HYBRIDIZING THE SCAFFOLD OF THE ESCHERICHIA COLI ENZYME WITH THE ACTIVE SITE LOOPS FROM THE THERMOANAEROBACTER TENGCONGENSIS ENZYME

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Phosphoenolpyruvate-protein phosphotransferase	polymer	316	35468.7	2	UniProt (A0A4S4CN20) Pfam (PF02896) UniProt (by SIFTS) (P08839)	Escherichia coli	Phosphotransferase system,enzyme I
2	C, D (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
3	E, F (A, B)	water	water		18.0	578	Chemie (HOH)

Sequence modifications

A, B: 261 - 575 (UniProt: A0A4S4CN20)

PDB	External Database	Details
Met 260	-	initiating methionine
Pro 278	Val 253	engineered mutation
Lys 279	Arg 254	engineered mutation
Tyr 301	Phe 276	engineered mutation
Asn 305	Asp 280	engineered mutation
Ser 306	Ala 281	engineered mutation
Ser 309	Thr 284	engineered mutation
Leu 334	Met 309	engineered mutation
Leu 345	Met 320	engineered mutation
Asp 346	Asn 321	engineered mutation
Met 347	Phe 322	engineered mutation
Met 351	Glu 326	engineered mutation
Tyr 357	Trp 332	engineered mutation
Met 466	Gly 441	engineered mutation
Glu 468	Asp 443	engineered mutation
His 469	Met 444	engineered mutation
Val 470	Ile 445	engineered mutation
Lys 471	Ser 446	engineered mutation
Glu 472	His 447	engineered mutation
Tyr 473	Leu 448	engineered mutation
Phe 477	Met 452	engineered mutation
His 478	Ser 453	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	70937.4
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	70986.0

*Water molecules are not included.