6V8G

GltPh mutant - Y204L A345V V366A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Glutamate transporter homolog	polymer	422	44591.9	3	UniProt (O59010) Pfam (PF00375) In PDB	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	Glt(Ph),Sodium-aspartate symporter Glt(Ph),Sodium-dependent aspartate transporter
2	A, B, C	SODIUM ION	non-polymer		23.0	9	Chemie (NA)
3	A, B, C	ASPARTIC ACID	non-polymer		133.1	3	Chemie (ASP)

Sequence modifications

A, B, C: 1 - 419 (UniProt: O59010)

PDB	External Database	Details
His 37	Asp 37	conflict
His 40	Lys 40	conflict
His 125	Lys 125	conflict
His 132	Lys 132	conflict
Leu 204	Tyr 204	engineered mutation
His 223	Lys 223	conflict
His 264	Lys 264	conflict
Val 345	Ala 345	engineered mutation
Ala 366	Val 366	engineered mutation
His 368	Glu 368	conflict
Thr 418	Glu 418	conflict
Val 420	-	expression tag
Pro 421	-	expression tag
Arg 422	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	133775.8
Non-Polymers*	Number of molecules	12
	Total formula weight	606.2
All*	Total formula weight	134382.0

*Water molecules are not included.