6V8G

GltPh mutant - Y204L A345V V366A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99997
Spacegroup name	P 31 2 1
Unit cell lengths	109.416, 109.416, 305.227
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.380 - 3.380
R-factor	0.2336
Rwork	0.232
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nwx
RMSD bond length	0.002
RMSD bond angle	0.453
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.380	3.496
High resolution limit [Å]	3.375	3.375
Rmerge	0.115
Rmeas	0.121
Rpim	0.036
Number of reflections	30612	2964
<I/σ(I)>	10.87
Completeness [%]	99.5
Redundancy	10.5
CC(1/2)	0.993	0.787

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	Potassium citrate, PEG 400